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内容説明 This widely expanded second edition offers a compilation of robust， reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published. The book begins with a discussion of long term ex situ conservation of biological resources， the role of biological resource centers， and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials， including proteins， mircroorganisms， cell lines， and multicellular structures.