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内容説明 A cutting-edge collection of diverse readily reproducible techniques for dissecting the molecular mechanisms of pain transduction. These methods employ a variety of multidisciplinary approaches ranging from animal pain models and single neutron selection to in vitro single-cell mRNA amplification. The collection includes not only standard and cutting-edge methods， but also novel techniques only recently applied to pain research. The protocols follow the successful Methods in Molecular BiologyTM series format， each one offering step-by-step laboratory instructions， an introduction outlining the principle behind the technique， lists of equipment and reagents， and tips on troubleshooting and avoiding known pitfalls.