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内容説明 A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands， including biochemical/bulk techniques， structure analysis， spectroscopy， single-molecule studies， and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches， high-throughput screening with confocal optics microscopy， single molecule fluorescence and fluorescence correlation spectroscopy (FCS)， atomic force microscopy (AFM)， crystallography of reaction intermediates， and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular BiologyTM series format， each offering step-by-step laboratory instructions， an introduction outlining the principle behind the technique， lists of the necessary equipment and reagents， and tips on troubleshooting and avoiding known pitfalls.