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内容説明
Macromolecular Crystallography Protocols， now in two volumes， examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1， Preparation and Crystallization of Macromolecules and Volume 2， Structure Determination， explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals， including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization， as well as computational techniques for data collection， phasing， and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.