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Book Description
High Performance Liquid Chromatography in Biotechnology Edited By William S. Hancock Analytical chemists， biochemists， or chemical engineers will find this up-to-date guide to HPLC’s recent developments essential to enhancing their on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC， ranging from major chromatographic techniques (including reversed phase， ion exchange， affinity and hydrophobic interaction chromatography) to specific separations such as in monoclonal antibody and nucleic acid purification. The book also highlights the techniques required for a quality control program and such advanced technology as mass spectrometry. 1990 (0 471-82584-0) 576 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together， for the first time in a single volume， the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physicalmathematical form， facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide generic background material applicable to a wide range of separation methods， including the theoretical foundations of separations rooted in transport， flow， and equilibrium phenomena. The final five chapters illustrate specific techniques and methods， including electrophoretic and sedimentation techniques， field-flow fractionation， and chromatography. 1991 (0 471-52089-6) 352 pp.
The publisher， John Wiley & Sons
The most popular separation technique used for trace and ultratrace analysis is high pressure/performance liquid chromatograph (HPLC). For the analyses of DNA and RNA， HPLC is proving extremely useful. Trace and ultratrace analyses could provide potentially helpful data for diagnostic studies. Deals with both analyses executed at or below ppm level and on-column injection at low microgram or nanogram level. For performance of trace and ultratrace analysis， a variety of applications， studies and methods are focal points for discussion， along with theoretical and equipment considerations.